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Table 1 Quality Control Metrics from DNA Workbench

From: CGH and SNP array using DNA extracted from fixed cytogenetic preparations and long-term refrigerated bone marrow specimens

Specimen Age of specimen1 Test Fluorochrome Pass/fail DLRSpread2 Signal to Noise 3 Green Signal to Noise 3 Red
bone marrow 1 day CY3 Pass 0.206803 65.848474 63.860383
   CY5 Pass 0.199919 62.305342 52.162212
bone marrow 9 days CY3 Pass 0.14503 98.418512 76.505274
   CY5 Pass 0.142963 87.635554 88.5432
bone marrow 15 days CY3 Pass 0.209813 44.387109 34.835231
   CY5 Pass 0.205444 53.479156 41.008914
bone marrow 26 days CY3 Pass 0.283387 38.740122 43.751865
   CY5 Pass 0.257136 39.285321 42.250693
bone marrow 27 days CY3 Pass 0.158672 85.404253 76.821745
bone marrow 36 days CY3 Pass 0.181903 71.365826 75.92553
   CY5 Pass 0.192083 59.676813 56.174289
cytogenetic specimen 52 days CY3 Pass 0.174283 123.04883 104.706454
cytogenetic specimen 1 year CY3 Pass 0.333462 40.65296 49.663864
   CY5 Pass 0.193984 56.378169 43.05297
cytogenetic specimen 8 years CY3 Pass 0.218933 47.366763 47.266465
   CY5 Pass 0.198984 57.315046 56.775467
  1. DNA Workbench quality classifications are given below the table. Quality measurements classified as "excellent" are in bold print and the single "poor" measurement is underlined; all other measurements were classified "good". Pairs of duplicate experiments are grouped together.
  2. 1 length of time at 4°C for bone marrow or at -80°C for chromosome suspension in Carnoy's fixative.
  3. 2 Excellent: < 0.2; Good: 0.2-0.3; Poor: > 0.3
  4. 3 Excellent: > 100; Good: 30-100; Poor: < 30