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Figure 4 | Molecular Cytogenetics

Figure 4

From: Centrosomal and mitotic abnormalities in cell lines derived from papillary thyroid cancer harboring specific gene alterations

Figure 4

Centrosome and mitotic spindle immunofluorescence, representative images. Centrosome γ-tubulin immunostaining (green signal): normal centrosome in NTHY-ORI 3-1 (a), TPC-1 (b) and K1 (c) cell lines; amplified centrosome in B-CPAP cell line (d). Centriol centrin 2 immunostaining (green signal): one pair of centriols in NTHY-ORI 3-1 (e), TPC-1 (f) and K1 (g) cell lines; amplification in B-CPAP cell line: four pairs (h) and five pairs (i) of centriols. Gamma- and β-tubulin immunostaining highlighting centrosomes (green signal) and mitotic spindle microtubules (red signal): normal mitotic figures in NTHY-ORI 3-1 (j), TPC-1 (k), and K1 (l) cell lines; abnormal mitotic figures in B-CPAP cell line: a tripolar metaphase (m), a quadripolar metaphase (n); a multipolar metaphase (o), and a tripolar metaphase with two acentrosomal spindle poles (p).

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Molecular Cytogenetics

ISSN: 1755-8166