FISH analysis for the confirmation of a-CGH results in the three de novo imbalances. A. In patient 1 the analysis performed using BAC probes RP11-742D12 in 18q21.1 (triplicated), RP11-160B24 in 18q21.2 (normal) and RP11-704G4 in 18q23 (normal). B. In patient 2 it was used BAC probe RP11-292F22 in 10q11.22 (duplicated) and cep10 probe (normal) C. Lastly in patient 3 BAC probes RP11-12N7 in 2q31.1 (normal) and RP11-358M9 in 2q31.3 (deleted) were applied.