Table 2 Identifiable differences of chromosome fragmentation and PCC
From: Comparison of mitotic cell death by chromosome fragmentation to premature chromosome condensation
Chromosome fragmentation | PCC |
|---|---|
Morphologic | |
Single cell involvement | If fusion induced, normal, intact mitotic cells will be in close proximity to fragmented cells |
Can affect single chromosomes | Impacts entire genome regularly, except in limited multinucleated cells |
Results in chromosome degradation | Unknown, may activate chromosome breakdown |
Chromosome morphology lost as process progresses | Chromosome morphology dependant on position in cell cycle |
Differential cut size | Differential condensation states |
Mechanistic | |
Occurs during mitosis | Occurs in interphase cells exposed to active MPF |
Not inhibited by roscovitine | Inhibited by roscovitine |
Induced by stress during mitosis | Induced by cell fusion or activation of MPF |
γ-H2AX positive | γ-H2AX negative |
No active DNA incorporation | Actively incorporating DNA |