Table 2 Overview of I-FISH techniques
From: Human interphase chromosomes: a review of available molecular cytogenetic technologies
| Technique | Brief description | Advantages | Limitations | Refs |
|---|---|---|---|---|
| I-FISH with centromeric probes | I-FISH on interpahse nuclei painting pericentromeric (heterochromatic) regions | High hybridization efficiency, chromosome specifity (apart few chromosomes) | Signal associations, impossible to analyze chromosomes 5,13, 14, 19, 21, 22; heteromorphisms | [7, 10, 20–22, 30, 35, 41, 58–60, 81, 82] |
| I-FISH with site-specific probes | I-FISH painting specific euchromatic genomic loci | Small specific genomic loci are visualized | Low hybridization efficiency, numerous artifacts | [8, 13, 28, 42, 47–50, 69] |
| I-FISH with wcp | I-FISH painting chromosome territories | Identification of nuclear chromosome territories | Chromosome territories are ambiguous, no additional information | [57, 70–72, 93] |
| mFISH | Multicolor I-FISH with >2 probes labeled by different fluorochromes/ligands | Analysis of several targeted genomic loci | Difficulty to distinguish between artifacts and aneuploidy/polyploidy | [7, 10, 20–22, 30, 35] |
| mFISH/QFISH | mFISH + QFISH digitalization of FISH signals | Distinguishes between FISH artifacts and aneuploidy (polyploidy) | Same as mFISH | [7, 10, 24–29, 32, 35] |
| MFISH | Simultaneous visualization of the complete set of chromosomes in an interphase nucleus | All chromosome territories are simultaneously seen | Exceedingly sophisticated analysis; data poorly interpretable | [70, 93] |
| ICS-MCB | Chromosome-specific MCB generated on interphase nuclei | Visualization of whole banded interphase chromosomes in their integrity | A pair of homologous chromosomes is studied per assay; relative complexity of the analysis | [7, 10, 13, 19, 23, 24, 26–29, 31, 33–35, 74, 75] |