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Table 2 Overview of I-FISH techniques

From: Human interphase chromosomes: a review of available molecular cytogenetic technologies

Technique Brief description Advantages Limitations Refs
I-FISH with centromeric probes I-FISH on interpahse nuclei painting pericentromeric (heterochromatic) regions High hybridization efficiency, chromosome specifity (apart few chromosomes) Signal associations, impossible to analyze chromosomes 5,13, 14, 19, 21, 22; heteromorphisms [7, 10, 2022, 30, 35, 41, 5860, 81, 82]
I-FISH with site-specific probes I-FISH painting specific euchromatic genomic loci Small specific genomic loci are visualized Low hybridization efficiency, numerous artifacts [8, 13, 28, 42, 4750, 69]
I-FISH with wcp I-FISH painting chromosome territories Identification of nuclear chromosome territories Chromosome territories are ambiguous, no additional information [57, 7072, 93]
mFISH Multicolor I-FISH with >2 probes labeled by different fluorochromes/ligands Analysis of several targeted genomic loci Difficulty to distinguish between artifacts and aneuploidy/polyploidy [7, 10, 2022, 30, 35]
mFISH/QFISH mFISH + QFISH digitalization of FISH signals Distinguishes between FISH artifacts and aneuploidy (polyploidy) Same as mFISH [7, 10, 2429, 32, 35]
MFISH Simultaneous visualization of the complete set of chromosomes in an interphase nucleus All chromosome territories are simultaneously seen Exceedingly sophisticated analysis; data poorly interpretable [70, 93]
ICS-MCB Chromosome-specific MCB generated on interphase nuclei Visualization of whole banded interphase chromosomes in their integrity A pair of homologous chromosomes is studied per assay; relative complexity of the analysis [7, 10, 13, 19, 23, 24, 2629, 31, 3335, 74, 75]