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Table 1 Molecular cytogenetic techniques, their resolution and validity for single-cell analysis of interphase/metaphase chromosomes (for more details see text)

From: Human interphase chromosomes: a review of available molecular cytogenetic technologies

Approach Resolution MA* IA** SCA^ PVC^^ Refs
Cytogenetic banding analysis ("golden standard") 5-7 Mb + - + + [1]
FISH/MFISH/SKY       
FISH/MFISH/SKY with centromeric probes >0.3-1 Mb +/- + + +/- [2026, 30, 32, 35, 41, 42, 44, 46]
FISH/MFISH/SKY with site-specific probes ~0.1-2 Mb +/- +/- +/- +/- [45, 4750]
FISH/MFISH/SKY with whole-painting probes >5-10 Mb + - + + [2, 3, 5, 6, 13, 36, 39, 40]
MCB       
Metaphase MCB ~2-5 Mb + - + + [2, 13, 43, 45]
ICS-MCB ~2-5 Mb - + + + [23, 24, 26, 28, 29, 3135]
Fiber FISH >2.3 (2-3) kb na na + + [51, 52]
Single-cell CGH       
Standard CGH 2-5 Mb na na + - [53]
Array CGH 0.03-1 Mb na na + - [14, 54]
  1. * -- analysis of metaphase chromosomes (MA - metaphase analysis); ** -- analysis of interphase chromosomes (IA - interphase analysis); ^ -- possibility to perform single cell analysis (SCA); ^^ -- possibility to visualize chromosomes or chromosomal loci (PVC - possibility to visualize chromosomal loci); na - not applicable;