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Table 1 Molecular cytogenetic techniques, their resolution and validity for single-cell analysis of interphase/metaphase chromosomes (for more details see text)

From: Human interphase chromosomes: a review of available molecular cytogenetic technologies

Approach

Resolution

MA*

IA**

SCA^

PVC^^

Refs

Cytogenetic banding analysis ("golden standard")

5-7 Mb

+

-

+

+

[1]

FISH/MFISH/SKY

      

FISH/MFISH/SKY with centromeric probes

>0.3-1 Mb

+/-

+

+

+/-

[2026, 30, 32, 35, 41, 42, 44, 46]

FISH/MFISH/SKY with site-specific probes

~0.1-2 Mb

+/-

+/-

+/-

+/-

[45, 4750]

FISH/MFISH/SKY with whole-painting probes

>5-10 Mb

+

-

+

+

[2, 3, 5, 6, 13, 36, 39, 40]

MCB

      

Metaphase MCB

~2-5 Mb

+

-

+

+

[2, 13, 43, 45]

ICS-MCB

~2-5 Mb

-

+

+

+

[23, 24, 26, 28, 29, 3135]

Fiber FISH

>2.3 (2-3) kb

na

na

+

+

[51, 52]

Single-cell CGH

      

Standard CGH

2-5 Mb

na

na

+

-

[53]

Array CGH

0.03-1 Mb

na

na

+

-

[14, 54]

  1. * -- analysis of metaphase chromosomes (MA - metaphase analysis); ** -- analysis of interphase chromosomes (IA - interphase analysis); ^ -- possibility to perform single cell analysis (SCA); ^^ -- possibility to visualize chromosomes or chromosomal loci (PVC - possibility to visualize chromosomal loci); na - not applicable;