Validation of DNA probes for molecular cytogenetics by mapping onto immobilized circular DNA

Table 1 Measurements of clone overlap

	vector		overlap		unique		total molecule
Image	pixel	kb	3015 (red)	kb	3012 (blue)	kb	pixel	kb(calc)
066.05.2	57.33	14.65	225.82	66.96	86.48	25.64	107.26	94.46
	56.47	14.43	224.75	67.66	86.06	25.91	108.00	93.86
	56.50	14.44	226.17	68.05	86.02	25.88	108.37	94.22
066.06.1	59.67	15.25	273.86	78.02	136.69	38.94	132.21	120.17
	60.76	15.53	273.85	76.62	136.52	38.20	130.34	120.40
	59.67	15.25	274.61	78.24	136.52	38.89	132.38	120.32
066.07.1	73.97	18.90	291.71	67.04	127.09	29.21	115.15	125.93
	73.64	18.82	292.46	67.52	127.60	29.46	115.79	126.17
	74.38	19.01	292.48	66.85	127.74	29.20	115.05	126.40
066.09.1	66.10	16.89	305.03	78.45	147.42	37.91	133.26	132.52
	65.03	16.62	306.61	80.15	149.41	39.06	135.83	133.16
	65.26	16.68	306.00	79.71	148.55	38.70	135.09	132.84
066.11.1	68.06	17.39	255.43	63.80	105.66	26.39	107.59	109.67
	68.05	17.39	255.34	63.79	104.11	26.01	107.19	109.25
	68.17	17.42	255.79	63.79	105.82	26.39	107.60	109.83
066.17.1	53.91	13.78	182.75	57.63	77.20	24.34	95.75	80.21
	53.74	13.73	182.24	57.65	76.99	24.35	95.74	79.98
	52.46	13.41	182.83	59.25	76.48	24.78	97.44	79.67
066.19.2	57.31	14.65	214.69	63.68	84.49	25.06	103.39	91.10
	56.08	14.33	212.95	64.55	83.69	25.37	104.25	90.14
	58.64	14.99	214.31	62.13	83.83	24.30	101.42	91.18
average		15.88		68.17		29.71
std. deviation		1.78		7.37		5.96

ISSN: 1755-8166