Figure 1
From: Validation of DNA probes for molecular cytogenetics by mapping onto immobilized circular DNA
P1 DNA molecules of different size. A) PFGE-analysis of the P1 clone RMC11P010. After incubation with Not1, pulse field gel electrophoresis showed circular DNA (c) and a band containing relaxed molecules (r) corresponding to a size of approximately 55 kb. The size marker lane ('szm', left) contains 500 ng of low range PFGE marker. B) Gel electrophoretic analysis of P1 clones containing part or all of the human NF-κB2 gene. The DNA from individual clones were digested with Not1 and separated by PFGE. All clones produced two or more restriction fragments indicating the presence of at least one Not1 site within the P1 insert. The size marker lane (szm) contains 500 ng of low range PFGE marker. C) Staining of gel-purified 'circular' P1 DNA molecules with YOYO-1 (Molecular Probes) reveals circular and randomly broken DNA molecules on the slide. Most of the DNA molecules shown here in the liquid phase prior to molecular combing are bound to the substrate with one end or via nicks in circular molecule.