Figure 3
From: Unusually stable abnormal karyotype in a highly aggressive melanoma negative for telomerase activity
Microsatellite instability in neoplasia (MIN) was excluded in this tumor since the microsatellite markers BAT-25 and BAT-26, showed no instability as compared to MIN positive and negative controls (A). The TRAP assay was negative for telomerase activity in cell culture material obtained from both peritoneal aspirations as compared to two well known telomerase positive human cancer cell lines (MCF-7 and HeLa) (B). Telomere length in this melanoma is relatively low as compared to the ALT U2-OS cell line, leukemic JURKAT cells and human embryonic fibroblasts (error bars represent standard error of the mean between 3 independent experiments) (C). Telomeric PNA FISH indicated uniform terminal capping with TTAGGG repeats on virtually all chromosomes in both pseudodiploid and endoreduplicated clones and low deviation of telomeric length in 500 chromatids as compared to the ALT U2-OS cell line (inverted DAPIx1000) (error bars represent standard deviation)(D).