FISH molecular testing in cytological preparations from solid tumors
© Caria and Vanni; licensee BioMed Central Ltd. 2014
Received: 12 May 2014
Accepted: 30 July 2014
Published: 22 August 2014
Many of the exciting new developments in solid tumor molecular cytogenetics impact classical and molecular pathology. Fluorescence in situ hybridization to identify specific DNA target sequences in nuclei of non-dividing cells in solid neoplasms has contributed to the integration of molecular cytogenetics into cytology in spite of the remarkable promiscuity of cancer genes. Indeed, although it is a low-throughput assay, fluorescence in situ hybridization enables the direct disclosure and localization of genetic markers in single nuclei. Gene fusions are among the most prominent genetic alterations in cancer, providing markers that may be determinant in needle biopsies that are negative or suspicious for malignancy, and may contribute to the correct classification of the tumors. In view of the expanding use of fluorescence in situ hybridization in cytology, future challenges include automated sample evaluation and the specification of common criteria for interpreting and reporting results.
KeywordsFISH Gene fusions Cytology Solid tumor Gene promiscuity
Various types of genetic alterations, as well as epigenetic phenomena, have been identified and are now considered important in the classification, prognosis, and treatment of cancer. Correlations between genomic instability and carcinogenesis have been extensively investigated, leading to the recognition of an increasing number of genetic abnormalities as a tumor driving force. Currently, several molecular approaches are available to investigate tumor cell pathobiology at different levels (chromosome, gene, gene expression). The predominant approaches include immunohistochemistry, fluorescence in situ hybridization (FISH), polymerase chain reaction, array-based and omics-based techniques –. The integration of results obtained using these platforms has been invaluable in clarifying genetic alterations associated with cancer and in interpreting the key role of the impaired signaling pathways. Gene gains and losses and gene disruptions by chromosome translocation, inversion, or deletion have been recognized as playing a pathogenetic role in many cancers. These exciting new developments in solid tumor molecular cytogenetics impact classical and molecular pathology, and an increasing number of chromosome markers have been integrated into World Health Organization tumor classifications . Some of these markers are also relevant to selection of therapies targeting the protein products of gene fusions. In this scenario the impact of testing gene alterations by interphase FISH in material from needle biopsies and organic fluids has rapidly increased.
Promiscuity: a false dilemma?
Cytology and cytogenetics
Considering the introduction of systematic genomic testing for some tumors (such as lung and breast cancer) ,, the consequent need for a correct evaluation of ratio value in the presence of genetic heterogeneity , and the growing demand for FISH tests in fine needle aspirations and organic fluids, two main challenges for the future can be foreseen: the implementation of automated FISH evaluation and the specification of common criteria for interpreting and reporting FISH results in as many tumor types as possible. A significant impediment to evaluating the ever increasing numbers of clinical FISH tests requested is imposed by the labor intensive nature of the assay, as each test requires scoring numerous interphase nuclei by double blind observation. Automated FISH, with strictly established parameters for standardization, could partly overcome these issues, although automation has yet to be perfected . Specific recommendations and guidelines for FISH on tumors have been established within ACMG (American College of Medical Genetics and Genomics)  and E.C.A (European Cytogeneticists Association) . On the other hand, common objective interpretation criteria for FISH on cytological preparations, as well as quality control and quality assurance policies, remain limited ,, and require an extraordinary cooperative effort and interaction between cytogeneticists and cytologists. It would be desirable to convene expert advisory panels from scientific societies of clinical cytogeneticists and pathologists to establish evaluation criteria for the various tumors, based on expertise and a review of published literature, with a view to establishing common shared recommendations.
Many of the exciting new developments of molecular cytogenetics are having a profound impact on classical and molecular cytology. The growing demand for cytological FISH tests highlights the need for the specification of common criteria for interpreting and reporting FISH results, for quality control and quality assurance policies, and for possible implementation of automated FISH evaluation.
PC and RV participated in commentary design and wrote the manuscript. They read and approved the final manuscript.
We wish to express our gratitude to Daniela V Frau for providing us with Figure 4. Manuscript preparation was funded by Fondazione Banco di Sardegna, grant prot No 17741 and POR Sardegna-FSE 2007–2013.
- Cai WW, Mao JH, Chow CW, Damani S, Balmain A, Bradley A: Genome-wide detection of chromosomal imbalances in tumors using BAC microarrays. Nat Biotechnol 2002, 20: 393–396. 10.1038/nbt0402-393View ArticlePubMedGoogle Scholar
- Maher CA, Kumar-Sinha C, Cao X, Kalyana-Sundaram S, Han B, Jing X, Sam L, Barrette T, Palanisamy N, Chinnaiyan AM: Transcriptome sequencing to detect gene fusions in cancer. Nature 2009, 458: 97–101. 10.1038/nature07638PubMed CentralView ArticlePubMedGoogle Scholar
- Hanash S, Taguchi A: The grand challenge to decipher the cancer proteome. Nat Rev Cancer 2010, 10: 652–660. 10.1038/nrc2918View ArticlePubMedGoogle Scholar
- Mainini V, Pagni F, Garancini M, Giardini V, De Sio G, Cusi C, Arosio C, Roversi G, Chinello C, Caria P, Vanni R, Magni F: An alternative approach in endocrine pathology research: MALDI-IMS in Papillary Thyroid Carcinoma. Endocr Pathol 2013, 24: 250–253. 10.1007/s12022-013-9273-8View ArticlePubMedGoogle Scholar
- Kanagal-Shamanna R, Portier BP, Singh RR, Routbort MJ, Aldape KD, Handal BA, Rahimi H, Reddy NG, Barkoh BA, Mishra BM, Paladugu AV, Manekia JH, Kalhor N, Chowdhuri SR, Staerkel GA, Medeiros LJ, Luthra R, Patel KP: Next-generation sequencing-based multi-gene mutation profiling of solid tumors using fine needle aspiration samples: promises and challenges for routine clinical diagnostics. Mod Pathol 2014, 27: 314–327. 10.1038/modpathol.2013.122View ArticlePubMedGoogle Scholar
- World Health Organization classification of tumours of soft tissue and bone. IARC Press, Lyon; 2013.
- Turc-Carel C, Philip I, Berger MP, Philip T, Lenoir G: Chromosomal translocation (11; 22) in cell lines of Ewing’s sarcoma. C R Seances Acad Sci III 1983, 296: 1101–1103.PubMedGoogle Scholar
- Zucman J, Delattre O, Desmaze C, Plougastel B, Joubert I, Melot T, Peter M, De Jong P, Rouleau G, Aurias A, Thomas G: Cloning and characterization of the Ewing’s sarcoma and peripheral neuroepithelioma t(11;22) translocation breakpoints. Gene Chromosome Canc 1992, 5: 271–277. 10.1002/gcc.2870050402View ArticleGoogle Scholar
- ChimerDB 2.0-a knowledgebase for fusion genes updated. In, [http://biome.ewha.ac.kr:8080/FusionGene/Search.jsp]
- COSMIC: Catalogue Of Somatic Mutations In Cancer. In, [http://cancer.sanger.ac.uk/cancergenome/projects/cosmic/]
- Cantile M, Marra L, Franco R, Ascierto P, Liguori G, De Chiara A, Botti G: Molecular detection and targeting of EWSR1 fusion transcripts in soft tissue tumors. Med Oncol 2013, 30: 412. 10.1007/s12032-012-0412-8PubMed CentralView ArticlePubMedGoogle Scholar
- Dal Cin P, Qian X, Cibas ES: The marriage of Cytology and Cytogenetics. Cancer Cytopathol 2013, 121: 279–290. 10.1002/cncy.21270View ArticlePubMedGoogle Scholar
- Lindeman NI, Cagle PT, Beasley MB, Chitale DA, Dacic S, Giaccone G, Jenkins RB, Kwiatkowski DJ, Saldivar JS, Squire J, Thunnissen E, Ladanyi M: Molecular testing guideline for selection of lung cancer patients for EGFR and ALK tyrosine kinase inhibitors. Guideline from the College of American Pathologists, International Association for the Study of Lung Cancer, and Association for Molecular Pathology. Arch Pathol Lab Med 2013, 137: 828–860. 10.5858/arpa.2012-0720-OAPubMed CentralView ArticlePubMedGoogle Scholar
- Wolff AC, Hammond ME, Hicks DG, Dowsett M, McShane LM, Allison KH, Allred DC, Bartlett JM, Bilous M, Fitzgibbons P, Hanna W, Jenkins RB, Mangu PB, Paik S, Perez EA, Press MF, Spears PA, Vance GH, Viale G, Hayes DF: Recommendations for human epidermal growth factor receptor 2 testing in breast cancer: American Society of Clinical Oncology/College of American Pathologists clinical practice guideline update. Arch Pathol Lab Med 2014, 138: 241–256. 10.5858/arpa.2013-0953-SAPubMed CentralView ArticlePubMedGoogle Scholar
- Bernasconi B, Chiaravalli AM, Finzi G, Milani K, Tibiletti MG: Genetic heterogeneity in HER2 testing may influence therapy eligibility. Breast Cancer Res Treat 2012, 133: 161–168. 10.1007/s10549-011-1744-3View ArticlePubMedGoogle Scholar
- Pajor G, Kajtár B, Pajor L, Alpár D: State-of-the-art FISHing: automated analysis of cytogenetic aberrations in interphase nuclei. Cytometry 2012, 81: 649–963. 10.1002/cyto.a.22082View ArticlePubMedGoogle Scholar
- Mascarello JT, Hirsch B, Kearney HM, Ketterling RP, Olson SB, Quigley DI, Rao KW, Tepperberg JH, Tsuchiya KD, Wiktor AE: Working Group of the American College of Medical Genetics Laboratory Quality Assurance Committee. Section E9 of the American College of Medical Genetics technical standards and guidelines: fluorescence in situ hybridization. Genet Med 2011, 13: 667–675. 10.1097/GIM.0b013e3182227295View ArticlePubMedGoogle Scholar
- Hasting R, Bown N, Tibiletti MG, Debiec-Rychter M, Vanni R, Espinet B, van Roy N, Roberts P, van den Berg-de-Ruiter E, Bernheim A, Ylstra B, Schoumans J, Chatters S, Zemanova Z, Stevens-Kroef M, Simons A, Heim S, Salido M, Betts DR: Guidelines for Cytogenetic Investigations in Tumours. E.C.A Newsletter 2014, 34: 7–18. [http://e-c-a.eu] http://e-c-a.euGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.