Volume 7 Supplement 1

Proceedings of the International Conference on Human Genetics and 39th Annual Meeting of Indian Society of Human Genetics

Open Access

Association of miR-27a, miR-181a and miR-570 genetic variants with gallbladder cancer susceptibility on North indian population

  • Annapurna Gupta1,
  • Kiran L Sharma1,
  • Annu Yadav1,
  • Vijay Kumar2,
  • Sanjeev Misra2,
  • Ashok Kumar3 and
  • Balraj Mittal1
Molecular Cytogenetics20147(Suppl 1):P17

DOI: 10.1186/1755-8166-7-S1-P17

Published: 21 January 2014

Background

MicroRNAs are small endogenously expressed short non-coding RNAs. They appear to be critical regulators of tumor biology as their aberrant expression is well characterized in cancer progression. The role of microRNA is not fully understood in gallbladder carcinoma, so in present study we investigated the role of miR-27a, miR-181a and miR-570 genetic variants with gallbladder cancer (GBC) susceptibility.

Material and methods

In this case-control study, we evaluated the role of miR-27a, miR-181a and miR-570 genetic polymorphisms with GBC susceptibility in North Indian population. The present study included 515 GBC patients and 200 healthy controls from North India. Genotypes were determined by TaqMan probes. Statistical analysis was done by SPSS ver. 16. In silico analysis was performed using Bioinformatics tools (F-SNP, FAST-SNP).

Results

Logistic regression analysis showed no significant association of miR-27a, miR-181a and miR-570 genetic polymorphism with GBC susceptibility (P> 0.05). On stratifying our data on the basis of gall stone status, the [AG+GG] genotypes of miRNA rs895819 (A>G) were significantly associated with increased risk of GBC in patients without stone (p=0.003 OR=1.83 [(95%CI) 1.23-2.72]. The genetic risk by miR-27a, rs895819 (A>G) was also modulated by tobacco consumption as the heterozygotes (AG) were at higher risk p=0.005 OR=1.94 [(95%CI) 1.22-3.08]. However, there was no association of miR-181a and miR-570 polymorphisms with disease risk in subgroup analysis. In-silico analysis showed change in transcriptional regulation of miR-27a and miR-570 variations.

Conclusions

We found significant association of miRNA rs895819 A>G with gallbladder cancer risk through gallstone independent pathway and tobacco usage.

Authors’ Affiliations

(1)
Departments of Genetics, Sanjay Gandhi Post Graduate Institute of Medical Sciences (SGPGIMS)
(2)
Department of Surgical Oncology, KGMU
(3)
Surgical Gastroenterology, SGPGIMS

Copyright

© Gupta et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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