High rates of de novo 15q11q13 inversions in human spermatozoa
© Molina et al; licensee BioMed Central Ltd. 2012
Received: 19 October 2011
Accepted: 6 February 2012
Published: 6 February 2012
Low-Copy Repeats predispose the 15q11-q13 region to non-allelic homologous recombination. We have already demonstrated that a significant percentage of Prader-Willi syndrome (PWS) fathers have an increased susceptibility to generate 15q11q13 deletions in spermatozoa, suggesting the participation of intrachromatid exchanges. This work has been focused on assessing the incidence of de novo 15q11q13 inversions in spermatozoa of control donors and PWS fathers in order to determine the basal rates of inversions and to confirm the intrachromatid mechanism as the main cause of 15q11q13 anomalies.
Semen samples from 10 control donors and 16 PWS fathers were processed and analyzed by triple-color FISH. Three differentially labeled BAC-clones were used: one proximal and two distal of the 15q11-q13 region. Signal associations allowed the discrimination between normal and inverted haplotypes, which were confirmed by laser-scanning confocal microscopy.
Two types of inversions were detected which correspond to the segments involved in Class I and II PWS deletions. No significant differences were observed in the mean frequencies of inversions between controls and PWS fathers (3.59% ± 0.46 and 9.51% ± 0.87 vs 3.06% ± 0.33 and 10.07% ± 0.74). Individual comparisons showed significant increases of inversions in four PWS fathers (P < 0.05) previously reported as patients with increases of 15q11q13 deletions.
Results suggest that the incidence of heterozygous inversion carriers in the general population could reach significant values. This situation could have important implications, as they have been described as predisposing haplotypes for genomic disorders. As a whole, results confirm the high instability of the 15q11-q13 region, which is prone to different types of de novo reorganizations by intrachromatid NAHR.
KeywordsLow Copy Repeats Non-allelic Homologous Recombination 15q11q13 Inversions Spermatozoa Fluorescence in situ Hybridization
The human genome has been proven to be a highly dynamic structure, showing a great number of structural and copy-number variations . Four major mechanisms contribute to the genesis of variations: non-allelic homologous recombination (NAHR), non-homologous end joining (NHEJ), fork stalling and template switching (FoSTeS) and retrotransposition . The presence of segmental duplications or low-copy repeats (LCR) throughout the human genome plays a significant role in the formation of variation through NAHR [2, 3]. LCRs are DNA fragments longer than 1 Kb in size which share more than 90% of sequence identity between paralogous copies . They represent 5% of the human genome, and their interspersed nature and sequence identity provide a substrate for NAHR .
Examples of polymorphic inversions and the related genomic disorders reported to be caused as secondary rearrangements.
3q29 microdeletion syndrome
8p23 microdeletion syndrome
15q13.3 deletion syndrome
17q21.21 deletion syndrome
The human 15q11-q13 region is a highly dynamic segment involved in different rearrangements by NAHR. This region is flanked by five complex LCRs (LCR15-1, LCR15-2, LCR15-3, LCR15-4 and LCR15-5), clustering most of the breakpoints involved in chromosome reorganizations . LCRs of the 15q11-q13 region are built by duplications of the HERC2 gene/pseudogene which form blocks called END-repeats that are oriented both in direct or indirect orientations . Two major types of deletions have been reported: Class-I deletions involve a 6.6-Mb region delimitated by two breakpoints localized within the LCR15-1 and LCR15-3, and Class-II deletions, which have the proximal breakpoint within the LCR15-2 and the distal one in the LCR15-3, leading to a loss of 5.3 Mb of genetic material . It is well known that paternal 15q11q13 deletions are the major cause of Prader-Willi syndrome (PWS; OMIM 176270) , while maternal deletions cause Angelman syndrome (AS; OMIM 105830) .
Inversions of the 15q11-q13 region have been indirectly related to the occurrence of genomic disorders. In particular, Gimelli et al. (2003)  reported a 5.3-Mb heterozygote inv(15)(q11q13) (corresponding to the Class-II deletion segment) in a significant proportion of mothers with Angelman syndrome-affected children. Based on these findings, they suggested the existence of haplotypes at risk for the generation of secondary rearrangements (deletions and/or duplications) as was previously described in the 7q11.23 region . Strikingly, this inversion was also observed in heterozygosis in 9% of the control population. Other NAHR-based chromosomal rearrangements have been reported in this region that emphasize its instability: duplications , supernumerary marker chromosomes , triplications  or partial uniparental disomies .
Our group has recently reported that some PWS fathers produce significantly increased frequencies of spermatozoa carrying de novo 15q11q13 deletions, suggesting the presence of predisposing haplotypes for intra-chromatid NAHR events . As intra-chromatid NAHR events could also generate inversions, in the present work we have analyzed the frequency of de novo 15q11q13 inversions in spermatozoa from control donors and PWS fathers. The aim of this work is: 1) to determine the basal rate of de novo 15q11q13 inversions, and 2) to investigate whether the PWS fathers with an increase of deletions also show increases of inversions.
Clone selection and positioning
The positions of the selected probes were verified on metaphase spreads. Every single probe showed specific signals on the pericentromeric area of chromosome 15 (identified by DAPI banding), and no cross-hybridization was observed.
Only spermatozoa with a well-defined boundary were evaluated. Overlapping spermatozoa were discarded from the count.
Only spermatozoa with a clear distribution of two associated signals (in close proximity or overlapped) and one separated signal were considered informative. The separated signal must be separated from the others by at least a two-fold longer distance compared with the separation of the associated signals.
Our design implies that two probes would potentially change their positions (RP11-1122J3 and RP11-322N14) while the third probe remains in place (RP11-230M20). This allowed us to unequivocally discriminate between normal and inverted haplotypes. According to the distribution of signals in the informative sperm nuclei, the following genotypes were assigned:
Normal (Figure 2a): spermatozoa displaying an association of the two distal signals (orange and blue) and the proximal signal (green) located apart from the others.
Type-1 inversion (Figure 2b): spermatozoa displaying the proximal signal (green) associated with the most distal signal (blue), and the orange signal being located apart.
Type-2 inversion (Figure 2c): spermatozoa displaying the proximal signal (green) associated with the orange signal, and the blue signal being located apart.
The presence of normal and inverted haplotypes was confirmed by confocal laser- scanning microscopy (Figure 2).
The hybridization efficiency of every single probe in sperm nuclei was higher than 98%.
Sperm-FISH results in control donors
% ± SEM
71.45% ± 1.66
86.91% ± 1.10
3.59% ± 0.46
9.51% ± 0.87
Sperm-FISH results in PWS fathers
% ± SEM
73.78% ± 1.05
86.87% ± 1.02
3.06% ± 0.33
10.07% ± 0.74
A total of 10,292 informative sperm nuclei from the control population were analyzed (Table 2). The mean frequency of Type-1 inversions was 3.59%, ranging from 1.29% to 5.32%, with a standard error of the mean (S.E.M) of 0.46%. The mean frequency of Type-2 inversions was 9.51% (± 0.87% SEM), ranging from 6.47% to 14.06%.
In fathers of PWS individuals, a total of 16,545 informative sperm nuclei were analyzed (Table 3). The mean frequency of Type-1 inversions was 3.06% (± 0.33% SEM), ranging from 1.37% to 5.73%. The mean frequency of Type-2 inversions was 10.07% (± 0.73% SEM), ranging from 5.39% to 15.02%.
No significant differences were observed for either the frequencies of Type-1 or Type-2 inversions between the PWS fathers and the controls (P > 0.05) (Figure 3b). Nevertheless, individual comparisons showed higher incidences of inversions in four PWS fathers due to increases of Type-2 inversions: PW-5, PW-10 and PW-13 (P < 0.05), or increases in both types of inversions: PW-1 (P < 0.05) (Table 3) (Figure 3c). It deserves to be mentioned that significant increases of 15q11q13 deletions were previously reported in all these four individuals . No significant correlation was observed between the frequencies of 15q11q13 inversions and age (P > 0.05).
When considering the frequency of the different stable products resulting from intrachromatid NAHR events, that is, inversions and deletions (data previously reported ), no correlation was observed in either the control population (P = 0.121) or in the PWS fathers. However, a tendency to correlate (r = 0.69; P = 0.062) was observed in the PWS fathers.
Sperm-FISH analyses have been widely used for cytogenetic studies in spermatozoa  and have been mainly focused on evaluating the genetic reproductive risk of carriers of abnormal karyotypes  and infertile patients . Furthermore, and more recently, we have demonstrated the reliability of this methodology to assess the rates of deletions and duplications in spermatozoa . In all of these approaches, the chromosomal constitution of the sperm nuclei is inferred by the evaluation of the presence or absence of a given FISH signal.
In the present work, the potential of sperm-FISH to detect the inversions has been evaluated. Our results confirm that this approach is feasible and realistic if an accurate experimental design is used, taking into consideration the relation between the genetic distances and the physical distances of FISH signals in the interphase nucleus.
Our experimental design has led to the observation of two types of signal distributions: two plus one (2+1) and associations of three. As the images of the three signals in association could be the consequence of chromatin loops resulting from high-order packaging of interphase DNA, all of the nuclei showing this distribution were classified as uninformative and were discarded for haplotype evaluation. The 2+1 distributions were assigned to three different haplotypes: normal, Type-1 and Type-2 inversions. In any case, the two associated probes were always located closer than 1 Mb and the probe apart was separated by a distance longer than 5 Mb. It is well-known that the separation of interphase FISH signals increases as the genomic distance between the probes becomes larger, and this correlation is consistent for genomic separations up to 2 Mb . As a consequence, the three signals in our experimental design will appear as specific distributions of 2 associated dots plus 1 separated dot.
Types of 15q11q13 inversions
The results obtained point to the existence of two types of inversions that involve the segments corresponding to the reported Class-I and Class-II 15q11q13 deletions. Our data is in agreement with the existence of the 15q11q13 inversions between the LCR15-2 and 3 (Type-2) previously described by other authors . Furthermore, our experimental approach allowed us to describe a new inversion involving the segment between LCR15-1 and 3 (Type-1 inversion). As in the case of deletions, the most frequent inversion was the Type-2 inversion. Altogether, these data suggest that the LCR15-2 could harbor longer stretches of homology than LCR15-1 with the LCR15-3, making the first more susceptible to NAHR events as was previously inferred by Makoff and Flomen 
Susceptibility to generate 15q11q13 inversions
Unexpectedly high rates of 15q11q13 inversions were found in spermatozoa from the two populations studied. Two pieces of evidence indicate that these inversions are generated de novo: 1) Since the frequency of inversions was not close to 50% in any case, the possibility of having any constitutional heterozygote inversion carrier can be ruled out. 2) Another possibility is the presence of a mosaicism for the inversion restricted to the germ line. Two pieces of data make such a possibility unlikely. First, inversions are considered to be a predisposing haplotype that increases the risk of secondary rearrangements such as deletions and duplications. While the incidence of inversion was consistently high in all control and PWS fathers, most of them did not show increases of deletions. Second, if mosaicism is present in testicular tissue, we would expect that the degree would be different among individuals generating different percentages of sperm inversions. Instead, as mentioned above, the percentage of sperm inversions was consistent (Tables 2 and 3).
The high rate of 15q11q13 inversions in spermatozoa suggests that a certain percentage of heterozygous carriers are present among the general population. Taking into account the frequency of sperm inversions observed in the present study (Type-1 and Type-2 inversions: 13.1%) and assuming that the frequency of these inversions would be the same in oocytes, we can infer that the frequency of heterozygous carriers in the general population would be 22.6% (2 × 13/100 × 87/100). These results are in agreement with the 9% of inv(15)(q11q13) heterozygous carriers (4 out of 44 individuals) found in the control population assessed by Gimelli et al.(2003) . This situation has already been described in population studies analyzing other regions with similar features such as 7q11.23 and 8p23.1, where inversions were observed in 5.8% and 79%, respectively [24, 25]. In this sense, it seems likely that the frequency of inversions in regions with a genomic architecture characterized as being flanked by LCR could be as high as the one described in the 15q11-q13 region, and this would strengthen the hypothesis that the frequencies of inversions are underestimated within the great deal of structural variants of the human genome .
This situation might have important implications at a population level. As was previously suggested, the presence of an inverted chromosome in heterozygosity originates an unpaired region at pachytene making the region prone to misalignment and NAHR. Thus, the risk for a secondary rearrangement affecting the offspring could be increased [6, 12, 13]. Consequently, heterozygote carriers of any of these types of inversions could have an increased risk of transmission of 15q11q13 deletions in descendants.
Intrachromatid NAHR is the major NAHR mechanism
Results obtained in the present work showed higher frequencies of 15q11q13 inversions than the frequencies of deletions and duplications previously reported in controls and PWS fathers , thus indicating that intrachromatid NAHR is the most frequent mechanism. Some PWS fathers showed significantly increased rates of inversions, as compared with the control population (Table 3). Strikingly, all of them were previously reported to have significant increases of 15q11q13 deletions . These data point out the important participation of intrachromatid NAHR in the generation of 15q11-q13 anomalies. As a whole, our results suggest that individuals at risk (those showing significant increases of 15q11q13 anomalies in spermatozoa) have a disrupted intrachromatid NAHR mechanism, as only the products derived from this mechanism are significantly increased.
Since no constitutional heterozygous carriers of inversions were detected in any of the subjects analyzed, and different outcomes were found to be increased in spermatozoa of some PWS fathers (15q11q13 deletions or both 15q11q13 deletions and inversions), differences in the genomic architecture of the LCRs flanking this region might exist. Some authors have reported structural variation in the LCRs flanking some regions involved in genomic disorders [26, 27]. They suggested that some specific haplotypes within these LCRs could predispose those regions to misalignment between paralogous copies and thus predispose the region to NAHR, increasing the risk of transmission of genomic disorders. Our results suggest that, in fact, differences in the genomic architecture of the LCR15s predispose these individuals to intrachromatid NAHR, thus producing different rearrangements. Some individuals would show haplotypes with longer stretches of direct homology between paralogous LCRs, thus increasing the rates of deletions in spermatozoa, whereas other individuals might have haplotypes with higher degrees of direct and inverted homologies with their own paralogous copies, thus increasing both the rates of de novo deletions and inversions in spermatozoa. In this sense, the availability of methodologies that enable studying the genomic architecture of specific LCRs would allow for identifying possible predisposing haplotypes that would explain these differences and that would potentially increase the risk of transmission of 15q11q13 anomalies.
The high incidence of de novo 15q11q13 inversions in spermatozoa indicates that the incidence of heterozygous inversion carriers in the general population could reach significant values. As a whole, results confirm the high instability of the 15q11-q13 region, which is prone to different types of de novo reorganizations, mainly generated by intrachromatid NAHR events.
Semen samples from 10 control donors aged between 24 and 50, and 16 PWS fathers from 32 to 60 years of age were obtained. Donors were volunteers recruited from the general population. All subjects had normal karyotypes and were normozoospermic. To our knowledge, none of them had been exposed to genotoxic agents, and no history of chemotherapy, radiotherapy or chronic illness was recorded.
All subjects gave their informed consent in writing to participate in the study and the protocol was approved by the Institutional Ethics Committee of the Universitat Autònoma de Barcelona.
Samples were processed as described previously by our group . Briefly, the sperm fraction was resuspended in hypotonic solution (0.075 M KCl) for 30 minutes at 37°C and fixed in methanol:acetic acid (3:1). Spermatozoa were spread on a slide and kept at -20°C until processed.
Three BAC clones were selected using the resources of the Genome Browser database (UCSC Assembly; February 2009). All clones were obtained from the Children's Hospital Oakland Research Institute, BACPAC resources (Oakland, CA 94609 USA). BAC DNA extraction was performed using the QIAprep Miniprep kit (Qiagen GmbH; Hilden, Germany) following manufacturer's instructions.
BAC clones RP11-1122J3, RP11-322N14 and RP11-230M20 were fluorescently labeled with Spectrum Green-dUTPs (Abbott Molecular; Abbott Park, IL, USA), Spectrum Orange-dUTPs (Abbott Molecular) and DEAC-dUTPs (Perkin Elmer Inc; Boston, USA), respectively, by Nick Translation (Roche; Mannheim, Germany). Probes were mixed with 10 μg of Cot 1 DNA (Invitrogen; Carlsbad, USA), ethanol precipitated and resuspended in hybridization buffer (50% Formamide, 1xSSC and 10% dextran sulphate) (Abbott Molecular).
Probe positions were verified on lymphocyte metaphase chromosomes. Hybridization in spermatozoa was determined by the evaluation of 1,000 sperm nuclei per probe in three different FISH experiments.
Fluorescence in situ Hybridization on sperm (sperm-FISH)
Prior to hybridization, sperm nuclei were decondensed by slide incubation at 37°C in Tris buffer containing 25 mmol/ml dithiothreitol and 1% Triton X-100 for 45 minutes.
A triple-color FISH using the three BAC clones differentially labeled was performed following standard procedures. Briefly, probes were denatured at 80°C for 8 minutes and pre-annealed at 37°C for 15 minutes. Sperm nuclei were denatured at 73°C in 70% formamide in 2xSSC for 5 minutes. Hybridization was carried out by adding 5 μl of the corresponding probe mixture (200 ng of each probe) to the sperm preparation and incubating the slides in a moist chamber at 37°C for 48 hours. Post-hybridization washes were performed in 1xSSC with 0.3% NP-40 at 73°C followed by 2xSSC with 0.1% NP-40 at room temperature, for one minute in each solution. Slides were mounted with antifade solution (Abbott Molecular).
Analyses were performed using an Olympus BX-60 epifluorescence microscope equipped with a triple-band pass filter, and specific filters for Aqua, FITC and Cy3.
A minimum of 1,000 informative sperm nuclei were analyzed per sample.
Confocal Laser-Scanning Microscopy
To confirm the existence of normal and inverted haplotypes, 80 sperm-nuclei were captured and analyzed using a confocal-laser scanning TCS-SP5 AOBS microscope (Leica Microsystems, Heidelberg Gmbh; Mannheim, Germany).
Spectrum Orange fluorochromes were excited with the 561-nm line of a DPSS laser and observed in the red channel at an emission range of 569-nm to 671-nm. Spectrum Green fluorochromes were excited with the 405-nm line of a diode laser and viewed in the green channel at 502-nm to 551-nm. Finally, DEAC fluorochromes were excited with the 488-nm line of an Argon laser and observed in the blue channel at an emission range of 444-nm to 500-nm. Stacks of 16 to 20 sections every 0.3 μm were acquired.
Image combining and processing were performed with the IMARIS software package version 2.7 (Bitplane AG; Zürich, Switzerland).
Data obtained were statistically analyzed using SPSS version 15.0 (SPSS Inc; Chicago, IL, USA) under the advice of the statistical service of the Universitat Autònoma de Barcelona.
To assess the relationship between the two inverted haplotypes, the following analyses were performed:
The mean frequencies of Type-1 and Type-2 inversions were compared in both populations by a Wilcoxon test.
A Pearson's correlation test between the frequencies of the two inverted haplotypes was performed.
To assess the susceptibility of the 15q11-q13 region to generate inversions, the following statistical tests were performed:
Population level: The mean population frequency of 15q11q13 inversions were compared between controls and PWS fathers by means of the Mann-Whitney test.
Individual level: A Chi-square test comparing the inversion frequencies of every single PWS father with the mean frequency of inversions observed in the control population was performed.
Age effect: A Pearson's correlation test between the sum of inversions and the age of all subjects was performed.
To assess a possible relationship between the frequency of inversions and the frequency of deletions previously found by our group :
Spearman's correlations between the frequency of the total inversions (Type-1 + Type-2) and the frequency of deletions were performed for both the control population and the PWS fathers.
Differences and correlations were considered statistically significant when P < 0.05.
Authors would like to thank Dr. Joan Aurich (OneCell Systems) for his kind assistance in BAC processing and labeling protocols. This work was supported by the projects CF-180034 (Universitat Autònoma de Barcelona) and SAF-2010-22241 (Ministerio de Ciencia e Innovación). Oscar Molina is the recipient of a grant from the Universitat Autònoma de Barcelona (UAB/PIF2007). This manuscript has been proofread by Mr. Chuck Simmons, a native English-speaking university instructor of English.
- Zhang F, Gu W, Hurles ME, Lupski JR: Copy number variation in human health, disease, and evolution. Annu Rev Genomics Hum Genet 2009, 10: 451–481. 10.1146/annurev.genom.9.081307.164217PubMed CentralView ArticlePubMedGoogle Scholar
- Gu W, Zhang F, Lupski JR: Mechanisms for human genomic rearrangements. Pathogenetics 2008, 1: 4. 10.1186/1755-8417-1-4PubMed CentralView ArticlePubMedGoogle Scholar
- Stankiewicz P, Lupski JR: Structural variation in the human genome and its role in disease. Annu Rev Med 2010, 61: 437–455. 10.1146/annurev-med-100708-204735View ArticlePubMedGoogle Scholar
- Emanuel BS, Shaikh TH: Segmental duplications: an 'expanding' role in genomic instability and disease. Nat Rev Genet 2001, 2: 791–800.View ArticlePubMedGoogle Scholar
- Bailey JA, Eichler EE: Primate segmental duplications: crucibles of evolution, diversity and disease. Nat Rev Genet 2006, 7: 552–564.View ArticlePubMedGoogle Scholar
- Feuk L: Inversion variants in the human genome: role in disease and genome architecture. Genome Med 2010, 2: 11. 10.1186/gm132PubMed CentralView ArticlePubMedGoogle Scholar
- Shaw CJ, Lupski JR: Implications of human genome architecture for rearrangement-based disorders: the genomic basis of disease. Hum Mol Genet 2004, 13(Spec No 1):R57–64.View ArticlePubMedGoogle Scholar
- Amos-Landgraf JM, Ji Y, Gottlieb W, Depinet T, Wandstrat AE, Cassidy SB, Driscoll DJ, Rogan PK, Schwartz S, Nicholls RD: Chromosome breakage in the Prader-Willi and Angelman syndromes involves recombination between large, transcribed repeats at proximal and distal breakpoints. Am J Hum Genet 1999, 65: 370–386. 10.1086/302510PubMed CentralView ArticlePubMedGoogle Scholar
- Makoff AJ, Flomen RH: Detailed analysis of 15q11-q14 sequence corrects errors and gaps in the public access sequence to fully reveal large segmental duplications at breakpoints for Prader-Willi, Angelman, and inv dup(15) syndromes. Genome Biol 2008, 8: R114.View ArticleGoogle Scholar
- Butler MG, Fischer W, Kibiryeva N, Bittel DC: Array comparative genomic hybridization (aCGH) analysis in Prader-Willi syndrome. Am J Med Genet A 2008, 146: 854–860.View ArticleGoogle Scholar
- Online Mendelian Inheritance in Man[http://www.ncbi.nlm.nih.gov/omim]
- Gimelli G, Pujana MA, Patricelli MG, Russo S, Giardino D, Larizza L, Cheung J, Armengol L, Schinzel A, Estivill X, et al.: Genomic inversions of human chromosome 15q11-q13 in mothers of Angelman syndrome patients with class II (BP2/3) deletions. Hum Mol Genet 2003, 12: 849–858. 10.1093/hmg/ddg101View ArticlePubMedGoogle Scholar
- Osborne LR, Li M, Pober B, Chitayat D, Bodurtha J, Mandel A, Costa T, Grebe T, Cox S, Tsui LC, et al.: A 1.5 million-base pair inversion polymorphism in families with Williams-Beuren syndrome. Nat Genet 2001, 29: 321–325. 10.1038/ng753PubMed CentralView ArticlePubMedGoogle Scholar
- Repetto GM, White LM, Bader PJ, Johnson D, Knoll JH: Interstitial duplications of chromosome region 15q11q13: clinical and molecular characterization. Am J Med Genet 1998, 79: 82–89. 10.1002/(SICI)1096-8628(19980901)79:2<82::AID-AJMG2>3.0.CO;2-PView ArticlePubMedGoogle Scholar
- Dennis NR, Veltman MW, Thompson R, Craig E, Bolton PF, Thomas NS: Clinical findings in 33 subjects with large supernumerary marker(15) chromosomes and 3 subjects with triplication of 15q11-q13. Am J Med Genet A 2006, 140: 434–441.View ArticlePubMedGoogle Scholar
- Ungaro P, Christian SL, Fantes JA, Mutirangura A, Black S, Reynolds J, Malcolm S, Dobyns WB, Ledbetter DH: Molecular characterisation of four cases of intrachromosomal triplication of chromosome 15q11-q14. J Med Genet 2001, 38: 26–34. 10.1136/jmg.38.1.26PubMed CentralView ArticlePubMedGoogle Scholar
- Salavoura K, Kolialexi A, Sofocleous C, Kalaitzidaki M, Pampanos A, Kitsiou S, Mavrou A: Complex rearrangements of chromosome 15 in two patients with mild/atypical Prader Willi syndrome. Genet Couns 2008, 19: 219–224.PubMedGoogle Scholar
- Molina O, Blanco J, Vidal F: Deletions and duplications of the 15q11-q13 region in spermatozoa from Prader-Willi syndrome fathers. Mol Hum Reprod 2010, 16: 320–328. 10.1093/molehr/gaq005View ArticlePubMedGoogle Scholar
- UCSC Genome Bioinformatics[http://genome.ucsc.edu/]
- Martin RH: Cytogenetic determinants of male fertility. Hum Reprod Update 2008, 14: 379–390. 10.1093/humupd/dmn017PubMed CentralView ArticlePubMedGoogle Scholar
- Anton E, Vidal F, Blanco J: Role of sperm FISH studies in the genetic reproductive advice of structural reorganization carriers. Hum Reprod 2007, 22: 2088–2092. 10.1093/humrep/dem152View ArticlePubMedGoogle Scholar
- Sarrate Z, Vidal F, Blanco J: Role of sperm fluorescent in situ hybridization studies in infertile patients: indications, study approach, and clinical relevance. Fertil Steril 2009, 93: 1892–1902.View ArticlePubMedGoogle Scholar
- van den Engh G, Sachs R, Trask BJ: Estimating genomic distance from DNA sequence location in cell nuclei by a random walk model. Science 1992, 257: 1410–1412. 10.1126/science.1388286View ArticlePubMedGoogle Scholar
- Bosch N, Morell M, Ponsa I, Mercader JM, Armengol L, Estivill X: Nucleotide, cytogenetic and expression impact of the human chromosome 8p23.1 inversion polymorphism. PLoS One 2009, 4: e8269. 10.1371/journal.pone.0008269PubMed CentralView ArticlePubMedGoogle Scholar
- Hobart HH, Morris CA, Mervis CB, Pani AM, Kistler DJ, Rios CM, Kimberley KW, Gregg RG, Bray-Ward P: Inversion of the Williams syndrome region is a common polymorphism found more frequently in parents of children with Williams syndrome. Am J Med Genet C Semin Med Genet 2010, 154C: 220–228. 10.1002/ajmg.c.30258PubMed CentralView ArticlePubMedGoogle Scholar
- Cusco I, Corominas R, Bayes M, Flores R, Rivera-Brugues N, Campuzano V, Perez-Jurado LA: Copy number variation at the 7q11.23 segmental duplications is a susceptibility factor for the Williams-Beuren syndrome deletion. Genome Res 2008, 18: 683–694. 10.1101/gr.073197.107PubMed CentralView ArticlePubMedGoogle Scholar
- Carvalho CM, Lupski JR: Copy number variation at the breakpoint region of isochromosome 17q. Genome Res 2008, 18: 1724–1732. 10.1101/gr.080697.108PubMed CentralView ArticlePubMedGoogle Scholar
- Sarrate Z, Anton E: Fluorescence in situ hybridization (FISH) protocol in human sperm. J Vis Exp 2009.Google Scholar
- Antonacci F, Kidd JM, Marques-Bonet T, Ventura M, Siswara P, Jiang Z, Eichler EE: Characterization of six human disease-associated inversion polymorphisms. Hum Mol Genet 2009, 18: 2555–2566. 10.1093/hmg/ddp187PubMed CentralView ArticlePubMedGoogle Scholar
- Visser R, Shimokawa O, Harada N, Kinoshita A, Ohta T, Niikawa N, Matsumoto N: Identification of a 3.0-kb major recombination hotspot in patients with Sotos syndrome who carry a common 1.9-Mb microdeletion. Am J Hum Genet 2005, 76: 52–67. 10.1086/426950PubMed CentralView ArticlePubMedGoogle Scholar
- Bayes M, Magano LF, Rivera N, Flores R, Perez Jurado LA: Mutational mechanisms of Williams-Beuren syndrome deletions. Am J Hum Genet 2003, 73: 131–151. 10.1086/376565PubMed CentralView ArticlePubMedGoogle Scholar
- Sugawara H, Harada N, Ida T, Ishida T, Ledbetter DH, Yoshiura K, Ohta T, Kishino T, Niikawa N, Matsumoto N: Complex low-copy repeats associated with a common polymorphic inversion at human chromosome 8p23. Genomics 2003, 82: 238–244. 10.1016/S0888-7543(03)00108-3View ArticlePubMedGoogle Scholar
- Sharp AJ, Mefford HC, Li K, Baker C, Skinner C, Stevenson RE, Schroer RJ, Novara F, De Gregori M, Ciccone R, et al.: A recurrent 15q13.3 microdeletion syndrome associated with mental retardation and seizures. Nat Genet 2008, 40: 322–328. 10.1038/ng.93PubMed CentralView ArticlePubMedGoogle Scholar
- Rao PN, Li W, Vissers LE, Veltman JA, Ophoff RA: Recurrent inversion events at 17q21.31 microdeletion locus are linked to the MAPT H2 haplotype. Cytogenet Genome Res 2010, 129: 275–279. 10.1159/000315901PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.