Unusually stable abnormal karyotype in a highly aggressive melanoma negative for telomerase activity
© Gagos et al; licensee BioMed Central Ltd. 2008
Received: 20 June 2008
Accepted: 22 August 2008
Published: 22 August 2008
Malignant melanomas are characterized by increased karyotypic complexity, extended aneuploidy and heteroploidy. We report a melanoma metastasis to the peritoneal cavity with an exceptionally stable, abnormal pseudodiploid karyotype as verified by G-Banding, subtelomeric, centromeric and quantitative Fluorescence in Situ Hybridization (FISH). Interestingly this tumor had no detectable telomerase activity as indicated by the Telomere Repeat Amplification Protocol. Telomeric Flow-FISH and quantitative telomeric FISH on mitotic preparations showed that malignant cells had relatively short telomeres. Microsatellite instability was ruled out by the allelic pattern of two major mononucleotide repeats. Our data suggest that a combination of melanoma specific genomic imbalances were sufficient and enough for this fatal tumor progression, that was not accompanied by genomic instability, telomerase activity, or the engagement of the alternative recombinatorial telomere lengthening pathway.
Cutaneous malignant melanomas are highly aggressive tumors with unpredictable biological behavior . Metastases in brain, bones and viscera with subsequent ascites development, are frequent . The progression of a transformed melanocyte to malignant melanoma is accompanied by gradual acquisition of multiple genetic alterations that lead to losses of onco-suppressor genes and increased tumor hypermutability . Malignant melanomas display both types of known genomic instability in neoplasia; chromosomal instability (CIN) and microsatellite instability (MIN) [2, 3]. MIN has been observed in 30% of cutaneous malignant melanomas . However, the great majority of malignant melanomas examined by various cytogenetic methods, exhibit increased karyotypic complexity, extended aneuploidy and heteroploidy [5–7]. Recurrent chromosomal imbalances in skin melanomas include losses of chromosomes 1p, 6q and 9p [2, 8]. Tumor progression and aggressive behavior have been associated with imbalances of chromosomes 7, 10 and 17 [2, 7].
Most human tumors including melanomas maintain sufficient telomere length for continuous growth by expressing telomerase [9, 10], the remainder are thought to utilize a variety of telomere recombination mechanisms termed alternative lengthening of telomeres (ALT) . Observations on transformed and tumor cell lines that lack telomerase, linked ALT phenotype to highly increased structural chromosomal instability and extreme telomeric length deviation ranging from very long to extremely short telomeres . We report a MIN, CIN, and TRAP negative (Telomere Repeat Amplification Protocol), highly aggressive melanoma metastasis to the peritoneal cavity, with unusually stable abnormal pseudodiploid karyotype, and relatively short but not dysfunctional telomeres.
Cell material from the peritoneal aspirations was subjected to routine diagnostic cytopathology protocols including Giemsa, Papanicolaou, Hematoxylin-Eosin (BDH-Chemicals) stains and immunocytochemistry using the melanocyte specific antibody S-100 (Dako). Immunocytochemical staining against S-100 was performed using Horse Radish Peroxidase (HRP) (Dako). Cell smears were re-hydrated, treated with 3% hydrogen peroxide for 15 minutes and rinsed with Tris Buffered Saline with 0.05% Tween 20 (Dako). After cooling for 20 min, sections were incubated with the primary antibody (rabbit antihuman monoclonal S-100 antibody, 1:400 dilution, Dako) for 1 hour at room temperature and then incubated for 45 min with an anti-mouse HRP labelled polymer (EnVision+System-HRP, Dako). Finally slides were treated with a diaminobenzidine (DAB) chromogenic substrate (Dako) for 10 min, counterstained with hematoxylin, dehydrated and coverslipped.
Short term cultures/Cytogenetic analysis
Malignant cells from two peritoneal aspirations were collected by centrifugation (10 min/1500 rpm/25°C). They were subsequently cultured in eight 25 cm2 T-flasks at 37°C and 5% CO2, in Dulbecco's Minimum Essential Medium supplemented with 10% fetal bovine serum, 0.08 mg/ml amphotericin, 25 units/ml penicillin, and 25 pg/ml of streptomycin (Invitrogen). When high mitotic index was reached, cells were exposed to colcemid (0.1 μg/ml) (Invitrogen) for 30 min, in 37°C, and harvested using trypsine (Invitrogen), after 0.075 KCL hypotonic treatment and Methanol/Acetic acid (BDH-Chemicals) fixation. For the construction of the representative karyotype, we combined G-Banding after Trypsine and Giemsa (GTG-Banding), inverted 4',6-diamidino-2-phenylindole (DAPI)-banding, subtelomeric FISH (TeloVysion-Vysis) and telomeric FISH (Dako), in a total of 400 metaphases from 8 short term monolayer cell cultures. For dual-color interphase or metaphase FISH we used satellite probes specific for chromosomes 7 and 17 (Cytocell). In brief, our general FISH protocol was based on pepsin pre-treatment, formamide or NaOH target denaturation, over-night hybridization and high stringency post hybridization washes. Telomere-specific Peptide Nucleic acid Analog (PNA) hybridizations were performed using a Cy3-(Indocarbocyanine)- conjugated (CCCTAA)3 probe (Dako), according to manufacturer's instructions. All FISH preparations were mounted and counterstained with VectaShield antifade medium (Vector), containing 0.1 μg/ml DAPI (Sigma). GTG-Banding was performed after trypsine denaturation (Invitrogen) and Giemsa (BDH-Chemicals) staining. Digital images were captured in a Perceptive Systems Imaging, a Metasystems or an Applied Imaging molecular cytogenetics workstations equipped with fluorescent Zeiss, or Nikon microscopes. Quantification of telomeric PNA fluorescence was performed in 500 chromatids on DAPI counterstained metaphase preparations in a single hybridization experiment using the Isis software (Metasystems).
Microsatellite instability assay
Two mononucleotide markers, BAT-25 and BAT-26 were tested for microsatellite instability by radioactive PCR after Polyacrylamide Gel Electrophoresis (PCR-PAGE) assay, using the following primers: BAT25.1 (5'-TCGCCTCCAAGAATGTAAGT-3'), BAT25.2 (5'-TCTGCATTTTAACTATGGCTC-3'), BAT26.1 (5'-TGACTACTTTTGACTTCAGCC-3') and BAT26.2 (5'-AACCATTCAACATTTTTAACCC-3'). Experiment was monitored by controls for human microsatellite stability (normal genomic and MIN DNA from a patient with human Hereditary Non-Polyposis Colon Cancer – HNPCC-).
Telomerase activity of cell lysates was analyzed by the telomeric repeat amplification protocol (TRAP) assay with a TRAPeze Telomerase Detection kit (Intergen) according to manufacturer's instructions. Approximately 106 cells were harvested and lysed in 400 μl of 1× CHAPS (3-[(3-Cholamidopropyl)dimethylammonio]propanesulfonic acid, 3-[(3-Cholamidopropyl)-dimethylammonio]-1-propanesulfonate) lysis buffer [Tris-HCl 10 mM, pH 7.5; 1 mM EGTA (ethylene glycol tetraacetic acid), 1 mM MgCl2, 0.5% CHAPS 10% glycerol, DEPC (Diethylpyrocarbonate) treated water on ice for 30 min. Cell debris were spun down for 20 minutes at 12,000 r.p.m at 4°C. Each reaction was carried out by using 2 μl of supernatant, 1 μl of each primer, 0.5 μl of Taq-Polymerase (TAKARA), 10 μl of solution-Q (Qiagen), 5 μl of 10× buffer, 2 μl of dNTPs, in DEPC treated water in final volume of 50 μl. The primers used for the TRAP-assay PCR, were TS-5'-AATCCGTCGAGCAGAGTT-3' and Cxa-5'-GTGTAACCCTAACCCTAACCC-3'. The PCR program consisted first of an incubation at 30°C for 30 min and then in a thermocycler, 94°C for 2 min; 94°C for 30 s, 50°C for 25 s, 72°C for 30 s (33×); 72°C for 1 min. PCR products were electrophoresed in a 10% 19:1 acrylamide gel (Sigma)/0.5× TBE (Tris/Borate/EDTA) buffer using the mini protean II gel system (Biorad). Gels were stained with 2 μl of SYBR Green (Sigma) for 15 min at room temperature in 50 ml of TBE 0.5× buffer, and then exposed to UV light and visualized by a Kodak image acquisition station.
To measure cellular telomere length, short term cultured cells were hybridized in situ with a fluorescent telomere-specific peptide nucleic acid probe, according to manufacturer's protocol. Briefly, cells were washed in PBS, and re-suspended to 105 cells/100 μl of a hybridization mixture (Dako) containing 70% formamide and a telomere-specific FITC (Fluorescein isothiocyanate)-conjugated PNA probe. Control samples were re-suspended in hybridization solution without probe to obtain background fluorescence values. After hybridization, cells were spun down and washed twice with 4 ml PBS (Phosphate Buffered Saline) at 40°C for 10 min and finally re-suspended in PBS containing 0.1% Bovine Serum Albumin, 10 μg/ml RNase A (Roche) and 0.1 μg/ml propidium iodide (Calbiochem-Novabiochem). Cells were analyzed on a FACScan flow cytometer (Becton Dickinson) or stored at 4°C before analysis.
Patient history and ascitic fluid samples
Examination of factors related to chromosome stability
Metastatic transition in most human tumors is accompanied by a series of complex recurrent and stochastic chromosomal anomalies. These changes reflect the evolutionary pressure held by the cancer cells to bypass natural barriers and re-establish continuous growth into unrelated histopathologic environments [17, 18]. In this report, the karyotype of the primary tumor is not available, therefore the relative simplicity of genomic imbalances encountered in metastasis, permits only a hypothetical reconstruction of the chromosomal evolution of the disease. It has been proposed that melanomas develop through a mode of karyotypic evolution, common to both low and high complexity karyotypes . To become malignant, an apparently normal melanocyte of this patient underwent multiple karyotypic alterations involving breakpoints in at least 7 different chromosomes as well as chromosomal losses and non-disjunctions. Although we cannot define the temporal order of the recorded rearrangements, we postulate that the hemizygous deletions 6q23qter and 9p- might be early events in the chromosomal evolution of this melanoma. Translocations and deletions involving the q-arm of human chromosome 6 have been found in more than 80% of melanomas . According to Hoglund et al (2005) , deletions of the distal 6q should be considered early chromosomal lesions in melanomas. Moreover, the short arm of chromosome 9 is the site of several cell cycle regulators that have been linked with familial disease, or associated to melanoma progression and aggressive behavior . The gains of genomic material and the additional deletions involving 10p, 11q and 17p, were by-products of unbalanced chromatid separation of balanced translocations and the isochromosome formation. These more complex alterations might represent later events in the process of the karyotypic evolution of the disease. Chromosomes 10 and 11 are frequently lost in metastatic melanomas whereas chromosome 7 is frequently gained [2, 5, 7, 20, 21].
Rearrangements affecting the short arm of chromosome 17, where the p53 gene is located, have been implicated in the pathogenesis of malignant melanoma . It is interesting that although p53 deficiency has been related to increased rates of numerical chromosome instability or polyploidy , in this melanoma hemizygosity of p53 was not associated with continuous genomic instability. MIN tumors display extremely low rates of CIN . We ruled-out the possibility that this melanoma belonged to this type of tumors. We also ruled-out CIN in our specimens, since this metastatic pseudodiploid tumor was highly cytogenetically stable by all means examined. These results are compatible with those of Abdel-Rahman et al. 2001 and Fabarius et al. 2003, who observed that chromosomes of near-diploid cells are structurally much more stable than those of highly aneuploid counterparts [23, 24]. Perhaps, the rare, melanoma described here, is unusually stable, because it is near-diploid, in contrast to the majority of highly aneuploid genomically unstable melanomas.
The majority of human malignant melanomas and melanoma cell lines studied with the TRAP assay were found to express telomerase activity [10, 25]. Furthermore, telomerase activity has been connected to aggressiveness of melanomas . In continuous neoplastic growth, insufficiently protected telomeres tend to undergo end-to-end fusions and to produce numerous complex chromosome rearrangements such as dicentric chromosomes and inverted duplications [27–29]. No evidence of such lesions was found in our specimens. The transient stage of structural chromosomal instability in this case, equally involved subtelomeric, centromeric and genomic regions, and gave rise to translocations with canonical orientation. Surprisingly, this metastatic tumor was negative for telomerase activity. Moreover, no signs of recombinatorial telomere elongation were present  since flow FISH showed relatively short telomeres and PNA FISH displayed a uniform terminal capping of virtually all chromosomes of this melanoma with TTAGGG repeats.
The remarkable stability, and telomeric integrity of the metastatic tumor presented here, can be attributed either to transient telomerase activation, or the action of an unknown but efficient telomere restoration mechanism. However, we can not exclude the possibility that adequate telomeric length for clonal expansion and metastasis was already acquired by the cancer progenitor melanocyte. This assumption might correlate with the relatively young age of the patient. A thorough examination of a series of human osteosarcomas revealed a category of tumors that do not express telomerase activity and do not display any ALT-pathway characteristics . Interestingly these tumors showed low rates of CIN . A similar sub-category might be also encountered in melanomas. The exceptional case reported here, suggests that metastatic progression in this melanoma, was not accompanied by genomic instability, telomerase activity, or the engagement of the classical alternative recombinatorial telomere lengthening (ALT) pathway.
We thank M. Morris for providing reagents. This work was supported by the Biomedical Research Foundation of the Academy of Athens Greece (BRFAA), in a starting intramural funding and the Grant#05NON-EU-449 of the Greek Secretariat of Research of the Greek Ministry of Development to SG.
- Rhodes AR: Cutaneous melanoma and intervention strategies to reduce tumor-related mortality: what we know, what we don't know, and what we think we know that isn't so. Dermatol Ther 2006, 19: 50–69. 10.1111/j.1529-8019.2005.00056.xView ArticlePubMedGoogle Scholar
- Hussein MR: Genetic pathways to melanoma tumorigenesis. J Clin Pathol 2004, 57: 797–801. 10.1136/jcp.2003.015800PubMed CentralView ArticlePubMedGoogle Scholar
- Lengauer C, Kinzler W, Vogelstein B: Genetic instabilities in human cancers. Nature 1998, 396: 643–649. 10.1038/25292View ArticlePubMedGoogle Scholar
- Hussein MR, Sun M, Tuthill RJ, Roggero E, Monti JA, Sudilovsky EC, Wood GS, Sudilovsky O: Comprehensive analysis of 112 melanocytic skin lesions demonstrates microsatellite instability in melanomas and dysplastic nevi, but not in benign nevi. J Cutan Pathol 2001, 28: 343–350. 10.1034/j.1600-0560.2001.280702.xView ArticlePubMedGoogle Scholar
- Thompson FH, Emerson J, Olson S, Weinstein R, Leavitt SA, Leong SP, Emerson S, Trent JM, Nelson MA, Salmon SE, et al.: Cytogenetics of 158 patients with regional or disseminated melanoma. Subset analysis of near-diploid and simple karyotypes. Cancer Genet Cytogenet 1995, 83: 93–104. 10.1016/0165-4608(95)00057-VView ArticlePubMedGoogle Scholar
- Schulten HJ, Gunawan B, Otto F, Hassmann R, Hallermann C, Noebel A, Fuzesi L: Cytogenetic characterization of complex karyotypes in seven established melanoma cell lines by multiplex fluorescence in situ hybridization and DAPI banding. Cancer Genet Cytogenet 2002, 133: 134–141. 10.1016/S0165-4608(01)00584-2View ArticlePubMedGoogle Scholar
- Hoglund M, Gisselsson D, Hansen GB, White VA, Sall T, Mitelman F, Horsman D: Dissecting karyotypic patterns in malignant melanomas: temporal clustering of losses and gains in melanoma karyotypic evolution. Int J Cancer 2004, 108: 57–65. 10.1002/ijc.11558View ArticlePubMedGoogle Scholar
- Bastian BC, Olshen AB, LeBoit PE, Pinkel D: Classifying melanocytic tumors based on DNA copy number changes. Am J Pathol 2003, 163: 1765–1770.PubMed CentralView ArticlePubMedGoogle Scholar
- Kim NW, Piatyszek MA, Prowse KR, Harley CB, West MD, Ho PLC, Coviello GM, Wright WE, Weinrich SL, Shay JW: Specific association of human telomerase activity with immortal cells and cancer. Science 1994, 266: 2011–2015. 10.1126/science.7605428View ArticlePubMedGoogle Scholar
- Carvalho L, Lipay M, Belfort F, Santos I, Andrade J, Haddad A, Brunstein F, Ferreira L: Telomerase activity in prognostic histopathologic features of melanoma. J Plast Reconstr Aesthet Surg 2006, 59: 961–968. 10.1016/j.bjps.2006.01.022View ArticlePubMedGoogle Scholar
- Muntoni A, Reddel RR: The first molecular details of ALT in human tumor cells. Human Molecular Genetics 2005, 14: 191–196. 10.1093/hmg/ddi266View ArticleGoogle Scholar
- ISCN: An international system for human cytogenetic nomenclature. Edited by: Mitelman F. Basel: S. Karger; 1995.Google Scholar
- Papadopoulos N, Nicolaides NC, Liu B, Parsons R, Lengauer C, Palombo F, D'Arrigo A, Markowitz S, Willson JKV, Kinzler KW, Jiricny J, Vogelstein B: Mutations of GTBP in genetically unstable cells. Science 1995, 268: 1915–1917. 10.1126/science.7604266View ArticlePubMedGoogle Scholar
- Morales CP, Holt SE, Ouellette M, Kaur KJ, Yan Y, Wilson KS, White MA, Wright WE, Shay JW: Absence of cancer-associated changes in human fibroblasts immortalized with telomerase. Nat Genet 1999, 21: 115–118. 10.1038/5063View ArticlePubMedGoogle Scholar
- Baerlocher GM, Mak J, Tien T, Lansdorp PM: Telomere length measurement by fluorescence in situ hybridization and flow cytometry: tips and pitfalls. Cytometry 2002, 47: 89–99. 10.1002/cyto.10053View ArticlePubMedGoogle Scholar
- Scheel C, Schaefer KL, Jauch A, Keller M, Wai D, Brinkschmidt C, van Valen F, Boecker W, Dockhorn-Dworniczak B, Poremba C: Alternative lengthening of telomeres is associated with chromosomal instability in osteosarcomas. Oncogene 2001, 20: 3835–3844. 10.1038/sj.onc.1204493View ArticlePubMedGoogle Scholar
- Nowell PC: The clonal evolution of tumor cell populations. Science 1976, 194: 23. 10.1126/science.959840View ArticlePubMedGoogle Scholar
- Gagos S, Irminger-Finger I: Chromosome instability in neoplasia: chaotic roots to continuous growth. Int J Biochem Cell Biol 2005, 37: 1014–1033. 10.1016/j.biocel.2005.01.003View ArticlePubMedGoogle Scholar
- Hoglund M, Frigyesi A, Sall T, Gisselsson D, Mitelman F: Statistical behavior of complex cancer karyotypes. Genes Chromosomes Cancer 2005, 42: 327–341. 10.1002/gcc.20143View ArticlePubMedGoogle Scholar
- Hussein MR, Sun M, Roggero E, Sudilovsky EC, Tuthill RJ, Wood GS, Sudilovsky O: Loss of heterozygosity, microsatellite instability, and mismatch repair protein alterations in the radial growth phase of cutaneous malignant melanomas. Mol Carcinog 2002, 34: 35–44. 10.1002/mc.10047View ArticlePubMedGoogle Scholar
- Stark M, Hayward N: Genome-wide loss of heterozygosity and copy number analysis in melanoma using high-density single-nucleotide polymorphism arrays. Cancer Res 2007, 67: 2632–2642. 10.1158/0008-5472.CAN-06-4152View ArticlePubMedGoogle Scholar
- Liu G, Parant JM, Lang G, Chau P, Chavez-Reyes A, El-Naggar AK, Multani A, Chang S, Lozano G: Chromosome stability, in the absence of apoptosis, is critical for suppression of tumorigenesis in Trp53 mutant mice. Nat Genet 2004, 36: 63–68. 10.1038/ng1282View ArticlePubMedGoogle Scholar
- Abdel-Rahman WM, Katsura K, Rens W, Gorman PA, Sheer D, Bicknell D, Bodmer WF, Arends MJ, Wyllie AH, Edwards PA: Spectral karyotyping suggests additional subsets of colorectal cancers characterized by pattern of chromosome rearrangement. Proc Natl Acad Sci USA 2001, 98: 2538–2543. 10.1073/pnas.041603298PubMed CentralView ArticlePubMedGoogle Scholar
- Fabarius A, Hehlmann R, Duesberg PH: Instability of chromosome structure in cancer cells increases exponentially with degrees of aneuploidy. Cancer Genet Cytogenet 2003, 143: 59–72. 10.1016/S0165-4608(03)00003-7View ArticlePubMedGoogle Scholar
- Parris CN, Jezzard S, Silver A, MacKie R, McGregor JM, Newbold RF: Telomerase activity in melanoma and non-melanoma skin cancer. Br J Cancer 1999, 79: 47–53. 10.1038/sj.bjc.6690010PubMed CentralView ArticlePubMedGoogle Scholar
- Glaessl A, Bosserhoff AK, Buettner R, Hohenleutner U, Landthaler M, Stolz W: Increase in telomerase activity during progression of melanocytic cells from melanocytic naevi to malignant melanomas. Arch Dermatol Res 1999, 291: 81–87. 10.1007/s004030050387View ArticlePubMedGoogle Scholar
- Murnane JP, Sabatier L, Marder BA, Morgan WF: Telomere dynamics in an immortal human cell line. EMBO J 1994, 13: 4953–4962.PubMed CentralPubMedGoogle Scholar
- Gisselsson D, Pettersson L, Hoglund M, Heidenblad M, Gorunova L, Wiegant J, Mertens F, Dal Cin P, Mitelman F, Mandahl N: Chromosomal breakage-fusion-bridge events cause genetic intratumor heterogeneity. Proc Natl Acad Sci USA 2000, 97: 5357–5362. 10.1073/pnas.090013497PubMed CentralView ArticlePubMedGoogle Scholar
- de Lange T: Shelterin: the protein complex that shapes and safeguards human telomeres. Genes Dev 2005, 19: 2100–2110. 10.1101/gad.1346005View ArticlePubMedGoogle Scholar
- Johnson JE, Gettings EJ, Schwalm J, Pei J, Testa JR, Litwin S, von Mehren M, Broccoli D: Whole-genome profiling in liposarcomas reveals genetic alterations common to specific telomere maintenance mechanisms. Cancer Res 2007, 67: 9221–9228. 10.1158/0008-5472.CAN-07-1133View ArticlePubMedGoogle Scholar
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